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'2015年福建省普通高中毕业班质量检查理科综合能力 生物测试第I卷(选择题)1.下列实验材料选择不合理的是A.用人口腔上皮细胞观察细胞的吸水和失水B.用人成熟红细胞探究温度对有氧呼吸的影响C.用洋葱鳞片叶内表皮细胞观察线粒体的形态和分布D.用洋葱根尖分生区细胞探究影响染色体数目变化的因素2.下列关于免疫活性物质的叙述,正确的是A.B细胞在淋巴因子作用下快速产生大量的抗体B.青霉素是一种可导致过敏反应的免疫活性物质C.可用人工标记的抗体对机体组织中的抗原进行检测D.体液中的溶菌酶参与杀灭细菌的过程属于特异性免疫3.化学诱变剂EMS能使基因中的G烷基化,烷基化的G与T配对。CLH2基因控制合成叶绿素酶,该酶催化叶绿素分解。研究人员利用EMS处理野生型大白菜(叶片浅绿色)种子,获得CLH2基因突变的植株甲(叶片深绿色)和乙(叶片黄色)。下列叙述正确的是A.植株乙叶片呈黄色,说明其叶绿素酶失去活性·9·
B.若植株甲自交获得叶片浅绿色的植株.说明浅绿色为显性性状C.获得植株甲和乙,说明EMS可决定CLH2基因突变的方向D.EMS处理后,CLH2基因经两次复制可出现G一C替换为A一T的现象4.用35S标记T2噬菌体侵染大肠杆菌,相关叙述正确的是A.噬菌体增殖时,需利用大肠杆菌的核糖体合成逆转录酶B.噬菌体增殖时,遗传信息不能由mRNA传递给tRNAC.子代噬菌体不能检测到35S,说明DNA是遗传物质D.若改用14C标记噬菌体,也能示踪区分DNA和蛋白质5.为研究影响豌豆幼苗细胞线粒体耗氧速率的因素,按图示顺序依次向测定仪中加入线粒体及相应物质,测定氧气浓度的变化.结果如图(注:图中呼吸底物是指在呼吸过程中被氧化的物质)。下列分析正确的是A.加入的呼吸底物是葡萄糖B.过程①没有进行有氧呼吸第三阶段C.过程②比⑤耗氧速率低的主要原因是【H]不足D.过程④比③耗氧速率低的主要原因是呼吸底物不足·9·
26.(14分)为探究不同波长的光和CO2浓度对葡萄试管苗光合作用的影响,用40W的白色、红色和黄色灯管做光源,设置不同CO2浓度,处理试管苗。培养一段时间后,测定试管苗的净光合速率(净光合速率=真光合速率一呼吸速率),结果如图。(l)光合作用中,催化三碳化合物还原的酶存在于叶绿体的 中。(2)a点的净光合速率大于c点,从光合作用的角度分析,原因是 。c点的净光合速率大于b点,从呼吸作用的角度分析,原因是 (3)实验结果表明,大棚种植葡萄时,应选用 色塑料薄膜搭建顶棚。·9·
(4)为探究黄光培养条件下葡萄试管苗的叶绿素含量是否发生改变,提出实验思路如下:分别取 和黄光条件下培养的试管苗叶片,提取其中的色素并用 (试剂)分离,通过比较滤纸条上 的来判断叶绿素含量是否发生改变。27.(12分)长爪沙鼠是某草原鼠害的主要物种。若用灭鼠剂防治,长爪沙鼠种群数量会迅速恢复。为了提高防治的有效性,科研人员研究了不育剂EP-1对当地长爪沙鼠种群数量的影响。3月初在试验区投放不育剂饵料,对照区为自然状态;3月一10月间定期取样调查种群数量和幼体(0一1月龄)比例,研究期间长爪沙鼠迁人迁出情况不明显,忽略不计。其中幼体比例统计结果如图:·9·
(1)若投放灭鼠剂,有毒物质会沿着 渠道进入其他生物体内,影响生态安全。(2)调查长爪沙鼠的种群密度时,在0.5hm2的样地上,第一次捕获60只,标记后放回,第二次捕获的40只中有20只带标记。据此估算,样地上长爪沙鼠的种群密度为 只/h.m2。捕获过的长爪沙鼠难以再次被捕获,因此估算值偏 (3)雌性激素的分泌存在分级调节机制。EP-1含有雌性激素,可反馈作用于 (器官),使雌性长爪沙鼠血液中促性腺激素的含量显著降低。(4)与对照区相比,试验区长爪沙鼠种群的繁殖高峰出现的时间 、出生率总体水平 28.(14分)研究人员选择果皮黄绿色、果肉白色、果皮有覆纹的纯合甜瓜植株(甲)与果皮黄色、果肉橘红色、果皮无覆纹的纯合甜瓜植株(乙)杂交,F1表现为果皮黄绿色、果肉橘红色、果皮有覆纹。F1自交得F2,分别统计F2各对性状的表现及株数,结果如下表。·9·
(1)甜瓜果肉颜色的显性性状是 。(2)据表中数据 (填“能,,或“不能,’)判断两对基因(A和a,B和b)自由组合,理由是 (3)完善下列实验方案,证明果皮覆纹性状由2对等位基因控制。实验方案:让F1与植株 (填“甲”或“乙,’)杂交,统计子代的表现型及比例;预期结果:子代的表现型及比例为 。(4)若果皮颜色、覆纹两对性状遗传遵循基因自由组合定律,则理论上F2中果皮黄色无覆纹甜瓜约有 株。33.[生物—现代生物科技专题」(10分)研究人员在培育转bt基因抗虫番茄过程中,选用hpt(抗潮霉素基因)为标记基因。为了提高转基因番茄的安全性,在筛选成功后,用Cre酶(能特异识别loxp位点,切除loxp位点间·9·
的基因序列)切除hpt。相关技术流程如图。(l)步骤①中,为防止原番茄植株带有病毒,应选用作为外植体;培养时,常在培养基中加入2,4-D,其作用是。(2)步骤②将bt基因导入愈伤组织的最常用方法是(3)步骤③需在培养基中加入以初步筛选出转人bt基因的植株。(4)提取F1植株的基因组DNA,用hpt特异性引物进行PCR。其中,植株1和2的PCR产物电泳结果如上图,据此判断,植株为不具有hpt的抗虫番茄。·9·
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