迪康可吸收生物膜论文：迪康可吸收生物膜 屏障作用 肌腱粘连 内源性愈合 主动活动度 生物力学

【关键词】迪康可吸收生物膜屏障作用肌腱粘连内源性愈合主动活动度生物力学【英文关键词】Barrierfunctiontendonadhesionsendogenoushealingactivedegreebiomechanics迪康可吸收生物膜论文：川芎嗪注射液联合可吸收生物膜预防术后肌腱粘连的实验研究【中文摘要】通过实验研究,探讨川芎嗪注射液与可吸收生物膜联合应用预防肌腱及组织粘连的有效性。方法：从86只健康成年三黄鸡选取78只,雌雄各半,采用随机数字表的方法分为对照组(A组)、可吸收生物膜组(B组)、川芎嗪+可吸收生物膜组(C组),每组26只。参照Sungur 【关键词】迪康可吸收生物膜屏障作用肌腱粘连内源性愈合主动活动度生物力学【英文关键词】Barrierfunctiontendonadhesionsendogenoushealingactivedegreebiomechanics迪康可吸收生物膜论文：川芎嗪注射液联合可吸收生物膜预防术后肌腱粘连的实验研究【中文摘要】通过实验研究,探讨川芎嗪注射液与可吸收生物膜联合应用预防肌腱及组织粘连的有效性。方法：从86只健康成年三黄鸡选取78只,雌雄各半,采用随机数字表的方法分为对照组(A组)、可吸收生物膜组(B组)、川芎嗪+可吸收生物膜组(C组),每组26只。参照Sungur N的造模方法行第三足趾屈趾深肌腱游离并切断1/2,断端吻合修复造模。B、C组应用可吸收医用膜(成都迪康中科生物医学材料有限公司)包绕腱周组织,C组用川芎嗪注射液在肌腱吻合口周围注射,完全浸透可吸收生物膜。关闭伤口,无菌敷料包扎。术后2、4、6、8周取材,各组实验动物分别进行大体观察,生物力学测定和组织学检测,透射电镜观察,并对各组数据进行统计学分析。结果：(1)大体观察：术后2、4、6、8周肌腱粘连程度分级,B组、C组动物肌腱缝合处始终保持光滑；A组肌腱缝合处形成大量粗大粘连带。B组与C组比较有统计学差异(P<0.05),B组、C组与A组比较有显著统计学差异(P<0.01),B、C组粘连程度明显小于A组。(2)生物力学测定：关节屈曲角度：术后2、4、6、8周,各组关节屈曲角度比值间差异有显著性意义(P<0.01)；B、C组屈曲角度比值均大于同一时相的A组,差异有显著性意义(P<0.01),C组屈曲角度比值大于同一时相的B组,差异有统计学意义(P<0.05)。肌腱滑动距离：术后2、4、6、8周,三组肌腱滑动距离比值间差异均有统计学意义(P<0.05)。三个时相上C组均优于A组、B组,差异有显著性意义(P<0.01)。肌腱最大抗断裂载荷：术后2、4周,三组肌腱最大抗断裂载荷比值间差异均有统计学意义(P<0.05)。三个时相上B、C组均优于A组,差异有显著性意义(P<0.05)；C组和B组比较,差异无统计学意义(P>0.05)。术后6、8周各组间最大抗断裂载荷比值间差异无显著性意义(P>0.05)。(3)组织学检测：HE染色：术后2、4、6周,B、C组胶原纤维排列较A组整齐,淋巴细胞浸润不明显。术后8周：A、B、C组肌腱断端间差异不明显,均可见胶原纤维连接,其间可见成熟的腱细胞,排列规则,呈波浪状。Masson染色：术后2、4周C组胶原纤维排列规则,未见明显增生,B组显示大量的腱周成纤维细胞增生,胶原纤维增生明显并趋向规则排列,A组胶原纤维排列紊乱,胶原束成熟度不一；6、8周B、C组胶原纤维增多,排列规则,胶原束直径一致,A组则胶原纤维不规则,胶原束直径大小不一。(4)透射电镜观察：术后4周,B、C组较A组成纤维细胞数量多且分化活动明显,细胞器增多,胶原原纤维排列趋于规则。术后8周A组胶原排列紊乱,B组仍可见部分成纤维细胞呈现活跃,周围胶原纤维排列尚规则；C组成纤维细胞分化成熟,胶原纤维数量及排列均优于A、B组。结论：1.迪康可吸收生物膜与川芎嗪均可有效预防肌腱粘连；迪康可吸收生物膜与川芎嗪综合应用在肌腱粘连的防治中效果显著,其作用机制主要为屏障性物理阻隔作用,降低外源性愈合发生机率并加速肌腱内源性愈合,其使用方法简单且吸收性良好,是一种理想的预防肌腱粘连的新方法。2.迪康可吸收生物膜与川芎嗪综合应用预防肌腱粘连疗效确切,其操作方法简单、安全性较高,为防止组织粘连特别是肌腱领域开辟了新途径。【英文摘要】:Experimentalstudytoexploretheligustrazineliquidandcanabsorbthebiofilmincombinationtopreventtheeffectivenessofthetendonsandtissueadhesionforclinicaladhesions.Methods:78selectedfrom86healthyandadultsanhuang,usingarandomnumbertableisdividedintocontrolgroup(groupA),canabsorbthebiofilm(Bgroup),TMP+absorptionofbiologicalmembranegroup(Cgroup)(n=26).ModelingmethodofthethirdtoeofthelinereferenceSungurNflexordigitorumprofundustendonfreeandcutoff1/2,stumpanastomosisrepairmodeling.B,GroupCapplicationsabsorbablemedicalfilm(ChengduDucamintheDivisionofBiomedicalMaterialsCo.,Ltd.)surroundingthetendontissues,anastomoticCtetramethylpyrazineinjectioninthetendonaroundtheinjectioncompletelysoakedabsorbablebiofilm. Woundclosure,steriledressing.Drawn2,4,6and8weeksaftereachgroupofexperimentalanimalswereobservedforgrossbiomechanicaltestandhistology,transmissionelectronmicroscopy,andeachgroupdatawerestatisticallyanalyzed.Results:(1)generalobservation:2,4,6and8weeksafterthedegreeoftendonadhesionclassification,groupB,groupCanimalstendonsuturealwaysmaintainsmooth;groupAsutureformedalargenumberofthickadhesions,moresignificantdifference(P<0.05)ingroupBandgroupC,groupCandgroupA,statisticallysignificantdifferences(P<0.01),GroupBstatisticallysignificantdifferences(P<0.01)ingroupB,C,adhesionwassignificantlylessthaningroupAcomparedwithgroupADeterminationof(2)Biomechanics:flexionangle:2,4,6and8weekspostoperatively,theflexionangleratiowassignificant(P<0.01);groupB,C,flexionangleratioisgreaterthanthesamephasedifferencebetweengroupATherearesignificantdifferences(P<0.01),whilegroupB,groupCrelativetotheflexionangleratioisgreaterthanthesamedifferencewasstatisticallysignificant(P<0.05).Tendonslidingdistance:2,4,6and8weeksaftersurgery,thedifferencebetweenthethreegroupsoftendon slidingdistanceratiowerestatisticallysignificant(P<0.05).Onthethree-phaseCweresuperiortogroupA,groupB,thedifferencewassignificant(P<0.01).Maximumresistancetofractureloadintendon:experimentaltoeandthecontralateralnormaltoefractureresistanceloadandtheratiooftoseethetextinthetable.Thestatisticsshowed:2,4weeksaftersurgery,threegroupsoftendonlargestdifferencebetweentheratiooftheresistancetofractureloadwerestatisticallysignificant(P<0.05).ThreephasesontheB,GroupCwerebetterthangroupA,thedifferencewasstatisticallysignificant(P<0.05);GroupCandGroupB,thedifferencewasnotstatisticallysignificant(P>0.05).8weeksaftersurgery,maximumresistancetofractureloadratiodifferencewasnotsignificantsignificance(P>0.05).(3)histologicaldetection:HEstaining:2,4and6weeksaftersurgery,B,CcollagenfibersarrangedingroupAneat,lymphocyteinfiltrationwasnotobvious.After8weeks:A,B,C,tendonstumpnosignificantdifferencesarevisiblecollagenfibersthatconnect,duringwhichvisiblematuretendoncells,regularlyarranged,wavy.Massonstaining:4weeksaftersurgeryingroupCthecollagenfiberarrangementrules,nosignificantproliferationgroupB showalotoftendonweeksfibroblastproliferation,collagenfiberswassignificantlyandtendstotheregulararrangementofcollagenfibersAdisorderedarrangementofcollagendifferentbeammaturity.(4)transmissionelectronmicroscopy:4weeksaftersurgery,B,GroupCthanAnumberoffibroblastsanddifferentiationactivitiessignificantlyincreasedorganelles,collagenfibrilarrangementtendstorule.8weeksaftersurgeryingroupAcollagenderangement,groupBisstillvisiblepartintoshowingactivefibroblastsaroundthecollagenfibersstillrules;C,composedoffibercelldifferentiationandmaturation,thenumberandarrangementofthecollagenfibersaresuperiortogroupA,B.Conclusion:1.DucamcanabsorbBiomembraneandtetramethylpyrazinecanbeeffectiveinpreventingtendonadhesion;theDICONcanabsorbthecomprehensiveapplicationofBiomembraneandtetramethylpyrazinesignificanteffectinthepreventionoftendonadhesion,anditsmechanismforthephysicalbarriereffectofthebarrierresistance,reducetheexogenoushealingincidenceandacceleratetendonendogenoushealing,itsuseissimpleandgoodabsorbency,isanidealnewmethodforthepreventionoftendonadhesion.2Ducamcanabsorb thecomprehensiveapplicationofthebiofilmandLigustrazinepreventionoftendonadhesionefficacy,itsmethodofoperation,highsecurity,openedupnewavenuesforthepreventionoftissueadhesionespeciallytendonareas.【目录】川芎嗪注射液联合可吸收生物膜预防术后肌腱粘连的实验研究中文摘要5-8ABSTRACT8-10英文缩略词表11-12引言12-131材料与方法13-201.1实验动物131.2主要仪器131.3主要试剂131.4动物分组13-141.5动物造模141.6观察指标及检测方法与测定14-171.7组织学检测17-191.8透射电镜检测19-201.9数据结果处理202结果20-412.1一般情况202.2大体观察20-222.3关节屈曲角度22-252.4肌腱滑动距离25-292.5肌腱最大抗断裂负荷29-312.6组织学检查31-382.7透射电镜观察38-413讨论41-514结论51-52参考文献52-54附录综述54-64参考文献60-64附图64-66致谢66-67攻读学位期间主要研究成果及发表论文67